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Optimization and characterization of SA-modified liposome/mRNA complexes. (A, B) Particle sizes and ζ-potentials of SA@DOTAP/CHOL-mRNA and SA@DOTAP-mRNA were prepared with different mass ratios of mRNA to SA. (C–H) GFP expression images (left) and quantitative analysis (right) of SA@DOTAP/CHOL-GFP and SA@DOTAP-mRNA in DC 2.4 cells under 10% serum and serum-free conditions. Data are presented as mean ± SD (n = 3), ∗∗∗P < 0.01, scale bar = 200 μm.
