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PCR amplification of CpG islands. (A) 294 bp human hMLH1 promoter from colon cancer cell line RKO; (B) 605 bp human Cox-2 promoter from gastric cancer cell line MKN45. M1, puC18 DNA/HeaIII marker; M2, 1 kb DNA ladder (Gibco no. 15615-016); lanes 1 and 4, untreated DNA; lanes 2 and 3, bisulfite-modified template.
