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. 2002 Feb 1;30(3):e13. doi: 10.1093/nar/30.3.e13

Figure 3.

Figure 3

DHPLC chromatograms of ssPCR and PCR products of CpG islands in the Cox-2 promoter. The PCR products were heteroduplexed, then sized at 48°C and detected at the PDT. Open arrow, methylated fragments/peaks; closed arrow, ummethylated fragments/peaks. (A) Sizing of Cox2 PCR and ssPCR products from the AGS, HEK and MKN45 cell lines at 48°C. (B and C) Cox-2 ssPCR products detected at 53 and 55°C, the PDT for the unmethylated and methylated templates, respectively. (D) Cox-2 PCR products detected at 61°C.