Figure 3.

Neuroprotective effects of micelles on SH-SY5Y cells incubated with different neurotoxic conditions. (A) Viability of SH-SY5Y cells after incubation with 100 μmol/L H₂O₂. The cells were pre-treated with different preparations. Results are presented as mean ± SD (n = 3, ∗P < 0.05, ∗∗∗∗P < 0.0001); (B) The operation field and glass micropipette under arthroscopy of the whole-cell patch-clamp technique; in the center of the field, the glass microelectrode had sealed one cell. (C) Electrical activities of SH-SY5Y cells recorded by whole-cell patch-clamp with different compliance currents (600, 800 and 1000 pA); SH-SY5Y cells were cultured in 50 mmol/L glutamine to induce excitotoxicity and then treated with different preparations; (D) Flow cytometry analysis of excitotoxic cell apoptosis after treatment with different preparations; Positive signals of Annexin V-FITC with negative signals of propidium iodide (PI) (the fourth quadrant) directed the early apoptosis of SH-SY5Y cells; (E) Quantification of the cellular early apoptosis of SH-SY5Y cells analyzed by flow cytometry. Data are presented as mean ± SD (n = 3, ∗P < 0.05, ∗∗∗∗P < 0.0001); (F) ROS-staining of SH-SY5Y cells treated with different preparations against 50 mmol/L glutamine-induced excitotoxic injury (scale bar: 50 μm; green signal: DCFH-DA activated by ROS) (G) Quantitative cellular uptake of the targeted micelle (DPLB@LTG) in SH-SY5Y cells treated with glutamate (Glu)-stimulated [marked as Glu (+)] and even DHAA-inhibitive conditions. Results are calculated according to intracellular LTG and presented as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01).