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. 2002 Feb 1;30(3):e14. doi: 10.1093/nar/30.3.e14

Figure 6.

Figure 6

Dependence of repair events on the distance separating nick and mismatch. Each lane represents a repair assay performed with 50 µg of nuclear extract from the LoVo cell line, defective in hMSH2 and MR activity (25). Where indicated, 300 ng of purified MutSα (MSH2/MSH6) was added (even numbered lanes). The three substrates used were: T·G-10H, T·G-40H and a derivative of f1MR1 containing a T·G mismatch (7) with the same nick orientation (5′) so that HindIII could be used to assess mismatch correction in each case.