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. 2023 Feb 23;18(3):706–719. doi: 10.1016/j.stemcr.2023.01.012

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© 2023 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

GRN^−/− mbOrgs show higher synaptic density when compared with GRN^+/+ mbOrg

(A) Confocal images of mbOrg slices (GRN^+/+ or GRN^−/−) showing evenly distributed expression of AQP4 and DAPI (scale bar, 50 μm).

(B) Confocal images of mbOrg slices (GRN^+/+ or GRN^−/−) showing evenly distributed expression of MAP2 and DAPI (scale bar, 20 μm).

(C) IMARIS 3D reconstruction of PSD95 and synaptophysin (SYP) staining in 4-week-old mbOrg (scale bar, 10 μm) showing higher synaptic density in GRN^−/− compared with GRN^+/+ mbOrg.

(D, E) Western blot of mbOrg whole lysate showing higher expression on Synapsin1 (SYN1) in GRN^−/− compared with GRN^+/+ mbOrgs when normalized to PonceauS staining and its quantification (E) (n = 4, unpaired t test, two tailed, p < 0.05, each n represents approximately 50 mbOrgs and was repeated independently four times).

(F, G) Western blot of mbOrgs whole lysate showing higher expression on PSD95 in GRN^−/− compared with GRN^+/+ mbOrgs when normalized to PonceauS staining and its quantification (G) (n = 4, unpaired t test, two tailed, p < 0.05, each n represents approximately 50 mbOrgs and was repeated independently four times). For all graphs data are presented as mean ± standard error of the mean.