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. 2023 Mar 2;18(3):618–635. doi: 10.1016/j.stemcr.2023.02.001

Figure 6.

Figure 6

DAPT-treated human colonies give rise to insulin-expressing cells in insulin-dependent diabetic mice

(A–C) (A) Experimental diagram. Data were analyzed between day 90 and 120 post-transplantation for (B) blood glucose and (C) body weight, with individual mice separated by sham-operated control (black, n = 11), responder (red, n = 7) and non-responder (blue, n = 8) mice. Data represent median blood glucose or average body weight ± SEM.

(D) IP-GTT analysis on control (black, n = 5), responder (red, n = 6), and non-responder (blue, n = 5) mice. AUC was analyzed as mean ± SD.

(E) Human C-peptide in serum, expressed as relative C-peptide fold change between time 0 and 60 min post glucose challenge from control (black, n = 4), responder (red, n = 6), and non-responder (blue, n = 3) mice.

(F) Bright-field image of a kidney grafted with DAPT-treated human colonies. Grafted cells are outlined (white dashed line). Scale bars, 3 mm (left) or 1 mm (right).

(G) H&E staining of a kidney graft, with kidney tissue shown at the bottom of the image. Scale bar, 200 μm.

(H–K) IF staining of grafted cells with INS (green) (G and H), GCG (white) (H), UCN3 (white) (J), or SLC2A1 (white) (K). Scale bar, 20 μm. ∗p < 0.05, ∗∗p < 0.01. See also Figures S5 and S6.