Figure 7.

Human ducts are heterogeneous with a subset resembling progenitor-like cells

(A) scRNA-seq of dissociated adult human exocrine tissue identifies 6 distinct clusters.

(B) Violin plots of representative gene markers.

(C) The ductal cluster was re-analyzed by principal-component analysis and segregated into four unique clusters (0–3).

(D) Heatmap of cluster-specific genes identified in the four ductal clusters.

(E) IPA predicted up and downregulated pathways using DE genes from the four ductal clusters.

(F) Cells simultaneously expressing SOX9, PDX1, and NKX6.1 are 3.6% of the total cells, and only in the ductal cell population.

(G) Uniform Manifold Approximation and Projections (UMAPs) of the ductal cluster showing the expression of the SOX9, PDX1, or NKX6.1, with percent positive cells calculated in each ductal cluster.

(H) TP cells are enriched in ductal cluster 0.

(I) IPA-predicted upregulated pathways in TP cells.

(J) StemID cluster 4 cells (blue) are overlaid with TP cells (red) within the ductal cluster.

(K) IPA-predicted upregulated pathways in StemID cluster 4. See also Figure S7 and Data S1.