Phenotypic characteristics of primary-derived macrophages and macrophage cell lines. Flow cytometric mean percent positive and mean fluorescent channel (MFC) data for macrophage cell-surface markers F4/80, CD14, FcR, CD11b, CD18, CD11c, CD54, CD40, TLR-4, and MMR, ± the standard error (data are representative of at least three experiments, statistical significance of p ≤ 0.05 is indicated by an asterisk). Cells were cultured on TCPS for 24 h in serum containing media as described in Materials and methods section.