Fig. 11.

Intra-thalamic injection of DMOG or Nigericin eliminated the therapeutic effects of SGB on mechanical allodynia and anxiety and depression in CPSP rats. A The experimental timeline of surgical procedure and behavior tests. B Intra-thalamic injections of DMOG or Nigericin decreased the PWMT of the contralateral hindpaw and the area under the curve of the PWMT in ITC rats given SGB (n = 5, PWMT: group, F_3,16 = 9.517, day, F_4,64 = 59.90, group × day, F_12,64 = 3.019, ^#p < 0.05, ^##p < 0.01, ITC + SGB + DSMO vs ITC + SGB + DMOG, *p < 0.05, p < 0.001, ITC + SGB + DMSO vs ITC + SGB + Nig; AUC: F_3,16 = 14.57, ^##p < 0.01). C Intra-thalamic injections of DMOG or Nigericin decreased the PWMT of the ipsilateral hindpaw and the area under the curve of the PWMT in ITC rats given SGB (n = 5, PWMT: group, F_3,16 = 17.76, day, F_4,64 = 60.99, group × day, F_12,64 = 2.905, ^#p < 0.05, ^###p < 0.001, ITC + SGB + DMSO vs ITC + SGB + DMOG, *p < 0.05, **p < 0.01,***p < 0.001, ITC + SGB + DMSO vs ITC + SGB + Nig; AUC: F_3,16 = 29.48 ^###p < 0.001). D Representative track plot in the OFT. E DMOG and Nigericin decreased the time spent and traveled distance traveled in central area, and but had no effect on the total traveled distance in ITC rats given SGB (n = 5, time in central area: F_3,16 = 12.60, ^##p < 0.01; distance in central area: F_3,16 = 8.491, ^##p < 0.01; total distance: ns, no significance). F DMOG and Nigericin increased the latency to feed in the NSFT in ITC rats given SGB (n = 5, F_3,16 = 27.42, ^###p < 0.001). G DMOG and Nigericin increased the immobility time in the FST in ITC rats given SGB (n = 5, F_3,16 = 8.085, ^##p < 0.01). Data are expressed as mean ± SEM, one-way ANOVA followed by Tukey test