Fig. 3.
Intra-thalamic injection of HIF-1α siRNA or NLRP3 siRNA significantly prevented ITC-induced mechanical allodynia and anxiodepressive-like behaviors in rats. A The experimental timeline of surgical procedure and behavior tests. B Both HIF-1α siRNA and NLRP3 siRNA silenced the expression of HIF-1α and NLRP3 in total proteins of the peri-thalamic lesion sites in ITC rats. (n = 5, HIF-1α: F2, 12 = 143.9, ###p < 0.001 vs ITC + siRNA-NC; NLRP3: F2, 12 = 248.2, ###p < 0.001 vs ITC + siRNA-NC). C Temporal changes of PWMT in contralateral hindpaw after thalamic hemorrhagic stroke and the area under curve of the contralateral hindpaw PWMT (n = 5, PWMT: group, F2, 12 = 13.49, day, F4, 48 = 14.54, group × day, F8, 48 = 2.182, #p < 0.05, ##p < 0.01, ###p < 0.001 ITC + siRNA-NC vs ITC + HIF-1α-siRNA, *p < 0.05, ***p < 0.001, ITC + siRNA-NC vs ITC + NLRP3-siRNA; AUC: F2, 12 = 20.89, ###p < 0.001). D Temporal changes of PWMT in ipsilateral hindpaw after thalamic hemorrhagic stroke and the area under curve of the ipsilateral hindpaw PWMT (n = 5, PWMT: group, F2, 12 = 11.54, day, F4, 48 = 21.28, group × day, F12, 48 = 2.796, #p < 0.05, ##p < 0.01, ###p < 0.001, ITC + siRNA-NC vs ITC + HIF-1α-siRNA, *p < 0.05, **p < 0.01, ITC + siRNA-NC vs ITC + NL; RP3-siRNA; AUC: F2, 12 = 16.86, ##p < 0.01, ###p < 0.001). E Representative track plot in the OFT. F HIF-1α siRNA and NLRP3 siRNA increased the time spent and traveled distance traveled in central area, and but had no effect on the total traveled distance in the OFT (n = 5, time in central area: F2, 12 = 10.51, ##p < 0.01; distance in central area: F2, 21 = 9.466, #p < 0.05; total distance: ns, no significance). G HIF-1α siRNA and NLRP3 siRNA decreased the latency to feed in the NSFT (n = 5, F2, 12 = 24.43, ###p < 0.001). H HIF-1α siRNA and NLRP3 siRNA decreased the immobility time in the FST (n = 5, F2, 12 = 5.035, #p < 0.05). Data are expressed as mean ± SEM, one-way ANOVA followed by Tukey test