Fig. 4.

Intra-thalamic injection of YC-1 or MCC950 significantly prevented ITC-induced mechanical allodynia and anxiodepressive-like behaviors in rats. A The experimental timeline of surgical procedure and behavior tests. B Intra-thalamic injections of YC-1 and MCC950 increased the PWMT of the contralateral hindpaw and the area under the curve of the PWMT following ITC (n = 8, PWMT: group, F_{2, 21} = 6.30, day, F_{4, 84} = 94.81, group × day, F_{8, 84} = 7.687, ^###p < 0.001, ITC + YC-1 vs ITC + Veh, **p < 0.001, ITC + Veh vs ITC + MCC950 ; AUC: F_{2, 21} = 10.48, ^##p < 0.01). C Intra-thalamic injections of YC-1 and MCC950 increased the PWMT of the ipsilateral hindpaw and the area under the curve of the PWMT following ITC (n = 8, PWMT: group, F_{2, 21} = 6.485, day, F_{4, 84} = 81.73, group × day, F_{8, 84} = 8.051, ^###p < 0.001, ITC + YC-1 vs ITC + Veh, **p < 0.01, ***p < 0.001, ITC + Veh vs ITC + MCC950; AUC: F_{2, 21} = 10.70, ^##p < 0.01). D Representative track plot in the OFT. E YC-1 and MCC950 increased the time spent and traveled distance traveled in central area, and but had no effect on the total traveled distance in the OFT (n = 8, time in central area: F_{2, 21} = 21.06, ^###p < 0.001; distance in central area: F_{2, 21} = 13.42, ^###p < 0.001; total distance: ns, no significance). F YC-1 and MCC950 decreased the latency to feed in the NSFT (n = 8, W_{2, 11.78} = 31.52, ^###p < 0.001). G YC-1 and MCC950 decreased the immobility time in the FST (n = 8, F_{2, 21} = 7.689, ^#p < 0.05, ^##p < 0.01). Data are expressed as mean ± SEM, one-way ANOVA followed by Tukey test; Welch ANOVA followed by Tamhane’s T2 test