Fig. 6.

Intra-thalamic injection of YC-1 or MCC950 reduced ITC-induced local inflammation and oxidative stress in the peri-thalamic lesion sites in rats. A Representative western blots of HIF-1α, NLRP3, and Caspase-1, and cleaved caspase-1 expression in total proteins of the peri-thalamic lesion sites in ITC rats. B–E Quantitative summary result showed intra-thalamic injection of YC-1 or MCC950 reduced HIF-1α (B), NLRP3 (C), Caspase-1 (D), and cleaved caspase-1 (E) expression in the peri-thalamic lesion sites in ITC rats (n = 5, HIF-1α: F_2,12 = 48.89, ^##p < 0.01, ^###p < 0.001 vs ITC + Veh; NLRP3: F_2,12 = 18.71, ^##p < 0.01, ^###p < 0.001 vs ITC + Veh; n = 4, Caspase-1: F_2,9 = 20.56, ^##p < 0.01, ^###p < 0.001 vs ITC + Veh; cleaved caspase-1 p20: F_2,9 = 19.36, ^##p < 0.01, ^###p < 0.001 vs ITC + Veh). F ELISA result showed intra-thalamic injection of YC-1 or MCC950 reduced HIF-1α in the peri-thalamic lesion sites in ITC rats (n = 5, F_2,12 = 38.82, ^##p < 0.01, ^###p < 0.001 vs ITC + Veh). G–K ELISA results showed intra-thalamic injection of YC-1 or MCC950 reduced TNF-α, IL-1β, IL-6 and IL-33 expression, but had no effect on CCL-12 expression in the peri-thalamic lesion sites (n = 5, TNF-α: F_2,12 = 5.292, ^#p < 0.05 vs ITC + Veh; IL-1β: F_2,12 = 23.33, ^##p < 0.01 vs ITC + Veh; IL-6: F_2,12 = 9.249, ^#p < 0.05, ^##p < 0.01 vs ITC + Veh; IL-33: F_2,12 = 5.978, ^#p < 0.05 vs ITC + Veh). L ELISA result showed intra-thalamic injection of YC-1 or MCC950 reduced the expression of antioxidant factor MDA in the peri-thalamic lesion sites (n = 5, F_2,12 = 16.69, ^##p < 0.01, ^###p < 0.001 vs ITC + Veh). M ELISA result showed intra-thalamic injection of YC-1 or MCC950 increased the expression of oxidative factor SOD in the peri-thalamic lesion sites (n = 5, F_2,12 = 21.45, ^##p < 0.01, ^###p < 0.001 vs ITC + Veh). Data are expressed as mean ± SEM, one-way ANOVA followed by the Tukey test