Fig. 8.

ALDOC and ENO2 knock down reduces the sphere-forming ability of HCC827 and T47D cells. A qRT-PCR analyses of ALDOC and ENO2 in HCC827 3D_SM, HCC827 3D_FBS^low, T47D 3D_SM, and T47D 3D_FBS^low upon ALDOC and ENO2 silencing alone or in combination. B L-lactic acid production assessed in HCC827 3D_SM, HCC827 3D_FBS^low, T47D 3D_SM, and T47D 3D_FBS^low upon ALDOC and ENO2 silencing alone or in combination. C Cell viability of HCC827 3D_SM, HCC827 3D_FBS^low, T47D 3D_SM, and T47D 3D_FBS^low upon ALDOC and ENO2 silencing alone or in combination assessed by Cell titer-Glo 3D assay and expressed as relative light unit (RLU). D Representative images and relative histograms of tumor spheroids morphology and diameter of HCC827 3D_SM, HCC827 3D_FBS^low, T47D 3D_SM, and T47D 3D_FBS^low upon ALDOC and ENO2 silencing alone or in combination. All the experiments were carried out in triplicate and results are presented as mean ± SD. p-value: * < 0.05, ** < 0.01, *** < 0.001. ns: not significant