Table 1.
Identification results of the selected 1D-PAGE band and 2D-PAGE spots by ESI-QUAD-TOF mass spectrometry (MS/MS) analysis
| Spot ID | Protein name | GenBank Accession no | Theoretical pI/MW (kDa) | Observed pI/MW (kDa) | Sequence coverage (%) | emPAI | Fold change |
|---|---|---|---|---|---|---|---|
| 17 kDa protein band from 1D-PAGE | Uncharacterized protein LOC110648447 [H. brasiliensis] | XP_021658364.1 | 5.2/17.0 | -/17.0 | 56.9 | 5.25 | n/a |
| XP_021658366.1 | 5.1/16.6 | 62.0 | 3.96 | ||||
| Oxygen-evolving enhancer protein 3–2, chloroplastic-like [H. brasiliensis] | XP_021639605.1 | 9.5/24.4 | 29.9 | 1.51 | |||
| C1 | 17.3 kDa class I heat shock protein-like [H. brasiliensis] | XP_021675560.1 | 6.0/18.4 | 6.5/22.0 | 8.0 | 0.22 | -1.308 |
| C2 | Superoxide dismutase [Cu–Zn]-like [H. brasiliensis] | XP_021640614.1 | 5.6/15.4 | 6.4/18.0 | 8.0 | 0.26 | -1.779 |
| C3 | ATP synthase subunit D, mitochondrial-like [H. brasiliensis] | XP_021666672.1 | 5.1/19.8 | 5.3/26.5 | 29.0 | 3.20 | -2.597 |
| C4 | Universal stress protein PHOS34 [H. brasiliensis] | XP_021668720.1 | 5.7/18.3 | 6.0/20.0 | 18.0 | 0.47 | -1.847 |
| C5 | Putative cytochrome c oxidase subunit II PS17 (Fragments) [Pinus strobus] | PS17_PINST | 9.6/17.1 | 5.9/18.5 | 50.0 | 3.44 | -8.712 |
| N1 | Uncharacterized protein LOC110648447 [H. brasiliensis] | XP_021658364.1 | 5.2/17.0 | 4.7/17.7 | 32.5 | 1.29 | Undetectable in control treatment |
| XP_021658365.1 | 5.3/16.7 | 35.0 | 1.87 | ||||
| XP_021658366.1 | 5.1/16.6 | 35.0 | 1.87 | ||||
| N2 | Uncharacterized protein LOC110648447 [H. brasiliensis] | XP_021658364.1 | 5.2/17.0 | 4.9/17.3 | 60.0 | 4.25 | Undetectable in control treatment |
| XP_021658366.1 | 5.1/16.6 | 62.3 | 2.55 | ||||
| N3 | Uncharacterized protein LOC110648447 [H. brasiliensis] | XP_021658364.1 | 5.2/17.0 | 5.2/16.3 | 68.2 | 5.46 | Undetectable in control treatment |
| XP_021658365.1 | 5.3/16.7 | 82.1 | 4.42 | ||||
| XP_021658366.1 | 5.1/16.6 | 81.0 | 5.69 | ||||
| N4 | Aminotran_3 domain-containing protein [Cephalotus follicularis] | GAV65601.1 | 6.4/51.0 | 6.3/17.0 | 1.0 | 0.07 | Undetectable in control treatment |
| N5 | Uncharacterized protein LOC110648447 [H. brasiliensis] | XP_021658364.1 | 5.2/17.0 | 5.5/17.3 | 40.0 | 3.22 | + 2.959 |
| XP_021658365.1 | 5.3/16.7 | 24.5 | 0.87 | ||||
| XP_021658366.1 | 5.1/16.6 | 24.5 | 0.87 | ||||
| N6 | Uncharacterized protein LOC110648447 isoform X1 [H. brasiliensis] | XP_021658364.1 | 5.2/17.0 | 5.7/17.3 | 7.0 | 0.22 | + 2.913 |
| N7 | Metalloendoproteinase 5-MMP-like [H. brasiliensis] | XP_021637894.1 | 7.0/34.4 | 7.0/20.5 | 6.0 | 0.53 | Undetectable in control treatment |