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. 2023 Mar 21;16:28. doi: 10.1186/s13045-023-01426-4

Table 3.

Senescence-induced therapies and immunotherapy response in cancer

Senescence-inducing therapy type Therapy Cancer and model Senescence biomarkers Potential immune response Potential tumor response Reference
Radiotherapy radiotherapy osteosarcoma mouse model SA-β-gal, p16, p21, SASP (IL-6, CCL2/3/4/5) increase NKT activation ↑ [111]
radiotherapy NSCLC human cell line xenografts SA-β-gal, STING, NF-κB, L1, p21, SASP (IFN-β, IL-1α, IL-6) increase macrophage activation ↑ [112]
radiotherapy + PARPi (ex vivo in tumor cells) (1) melanoma; (2) PDAC syngeneic transplant mouse models SA-β-gal, p16, p21, SASP (CCL5, IFN-β, CXCL9/10/11) increase DC, CD8+ T, NK activation ↑ [113]
Chemotherapy cyclophosphamide B-cell lymphoma syngeneic transplant mouse model SA-β-gal, NF-κB, p15, SASP (IL-6, IL-8, ICAM-1, CXCL1) increase NK activation ↑ [114]
doxorubicin or melphalan MM syngeneic transplant mouse model SA-β-gal, p16, p53, NK ligands (RAE-1, MICA, MULT-1, PVR) increase NK activation ↑ [115]
cisplatin + irinotecan (ex vivo in tumor cells) ovarian cancer syngeneic transplant mouse models SA-β-gal, STING, p16, yH2AX, SASP (IL-6, VEGFA, GM-CSF) increase DC, CD8+ T activation ↑ [116]
docetaxel PCa GEMM SA-β-gal, p16, p21, SASP (GM-CSF, CSF-1, IL-10, CCL2, CXCL1/2) (1) increase MDSC activation ↑; (2) decrease NK, CD8+ T activation ↓ [117]
mitoxantrone, other agents (1) PCa human xenografts; (2) PCa clinical samples SA-β-gal, p16, SASP (IL-6, IL-8, MMPs, AREG), PD-L1 decrease CD8+ T activation ↓ [118]
Aurora kinase inhibitors MLN8054/MLN8237 (AURKAi) (1) melanoma human xenografts; (2) PDXs; (3) syngeneic SA-β-gal, NF-κB, SASP (IL-6, IL-8, CCL5, CXCL1/2) increase macrophage, CD8+ T activation ↑ [119]
MLN8237 (AURKAi) melanoma patient samples SASP (CCL5) increase CD8+ T activation ↑ unclear [119]
AZD1152 (AURKBi) (1) melanoma; (2) CRC syngeneic transplant mouse models SA-β-gal, p21 increase CD8+ T activation ↑ no significant change [120]
Cell Cycle inhibitors abemaciclib (CDK4/6i) (1) ER + breast cancer GEMM; (2) PDXs SA-β-gal, MHC-I (1) increase CD8+ T activation ↑; (2) decrease Treg response ↓ [121]
abemaciclib (CDK4/6i) melanoma syngeneic transplant mouse models SA-β-gal, SASP (CCL20, CX3CL1) release T-cell suppression ↓ no significant change [122]
palbociclib (CDK4/6i) + trametinib (MEKi) LUAD GEMM SA-β-gal, NF-κB, p15, SASP (TNF-α, ICAM-1, IL-15, NKG2D ligands) increase NK activation ↑ [36]
palbociclib (CDK4/6i) + trametinib (MEKi) PDAC GEMM SA-β-gal, SASP (VEGFs, PDGFs, MMPs, IL-6, CXCL1, CCL5), MHC-I, PD-L1 increase CD8+ T activation ↑ no significant change [37]
palbociclib (CDK4/6i) (ex vivo in fibroblasts) melanoma syngeneic transplant mouse models SA-β-gal, NF-κB, p16, SASP (IL-6, MMP3, CCL6, CCL8, CCL11) increase MDSCs activation ↑ [123]
XL413 (CDC7i) (1) HCC GEMM; (2) human xenografts SA-β-gal, p16 increase Mac, CD8+ T, CD4+ T activation ↑ [124]
Pro-senescence + Immunotherapy cisplatin + irinotecan (chemotherapy) + a-PD-1 ICI ovarian cancer syngeneic transplant mouse models SA-β-gal, STING, p16, yH2AX, SASP (IL-6, VEGFA, GM-CSF) increase CD8+ T, DC infiltration ↑ ↓↓ [116]
Mitoxantrone (chemotherapy) + a-PD-1 ICI PCa human xenografts SA-β-gal, p16, SASP (IL-6, IL-8, MMPS, AREG), PD-L1 increase CD8+ T infiltration ↑ [118]
MLN8237 (AURKAi) + a-CD137 (T cell agonist) melanoma syngeneic transplant mouse models SA-β-gal, NF-κB, SASP (IL-6, IL-8, CCL5, CXCL1/2) increase CD8+ T infiltration ↑ ↓↓ [119]
AZD1152 (AURKBi) + a-CTLA-4 ICI (1) melanoma; (2) CRC syngeneic transplant models SA-β-gal, p21 increase CD8+ T infiltration ↑ [120]
abemaciclib (CDK4/6i) + a-PD-1 ICI ER + breast cancer GEMM SA-β-gal, MHC-I increase CD8+ T infiltration ↑; decrease Treg activation ↓ ↓↓ [121]
abemaciclib (CDK4/6i) + a-PD-1/CTLA-4 ICI melanoma syngeneic transplant mouse models SA-β-gal, SASP (CCL20, CX3CL1) release T-cell suppression ↓ [122]
palbociclib (CDK4/6i) + trametinib (MEKi) + a-PD-1 ICI PDAC GEMM SA-β-gal, NF-κB, SASP (VEGFs, MMPs, PDGFs, IL-6, CXCL1, CCL5), MHC-I, PD-L1 increase CD8+ T activation ↑ [123]

SA-β-gal senescence-associated beta-galactosidase, SASP senescence-associated secretory phenotype, NSCLC non-small cell lung cancer, PDAC pancreatic ductal adenocarcinoma, NKT Natural Killer T cell, DC dendritic cell, NK Natural Killer cell, PCa prostate cancer, GEMM genetically engineered mouse model, MDSC myeloid-derived suppressor cell, AURKA Aurora Kinase A, AURKB Aurora Kinase B, PDX patient-derived xenograft, CRC colorectal cancer, ER estrogen receptor, HCC hepatocellular carcinoma, ICI immune checkpoint inhibitor, LUAD lung adenocarcinoma, MM multiple myeloma, i inhibitor (Mainly from 10.1016/j.semcancer.2022.02.005)