CE does not impact DNA methylation in spermatogenic cells at genes from the SFARI list without bivalent chromatin. In each panel, left graph shows methylation at individual CpG sites in SSC-like cells and right graph shows level in spermatid-like cells. Two-factor ANOVA for bisulfite pyrosequencing data shows no effect of CE exposure on DNA methylation at all genes analyzed, including A:
BRD4, B:
CADPS, C:
EXOC3, D:
FBXO40, E:
MED12L, F:
TSPAN17. Grey=controls, blue=CE exposed. ns=no significance