Table 1.

SFARI and bivalent chromatin gene assay information for bisulfite pyrosequencing

Gene	F Primer, 5'-3' (*biotin)	R Primer, 5'-3' (*biotin)	Sequencing Primer, 5'-3'	Sequence to Analyze, 5'-3'	Thermocycler Conditions
BRD4	BRD4-F	BRD4-R*	BRD4-S	BRD4	95°C 15 min
					94°C-63.5°C-72°C X 55 ∣30s
					72°C 10 min
	AGGTGGTTTTAGTTTTGAGTGTG	AAATTCAAAAAACACAAACCTCACATTAC	TGTTTTGTTTGTTTGGTTTAT	AGTTTAAAGTYGTGTAYGAG	4°C ∞
CADPS	CADPS-F	CADPS-R*	CADPS-S	CADPS	95°C 15 min
					94°C-68°C-72°C X 5 ∣30s
					94°C-66°C-72°C X 5 ∣30s
					94°C-64°C-72°C X 55 ∣30s
					72°C 10 min
	GTATAGGGGATAAAGTTTTTGTGG	AAAAAACCCTAAACTTCTATCTACTCCT	GATATTATATTGTTAGTGGGAAGTA	YGTTTAGAATTTATATGGYGAAAGGTTTGTTAATGAGTTGYGG	4°C ∞
EXOC3	EXOC3-F	EXOC3-R*	EXOC3-S	EXOC3	95°C 15 min
					94°C ∣30s
					63.5°C ∣30s X55
					72°C ∣30s
					72°C 10 min
	GTTTGGGAGAGTATGGGTGATAT	ACTCCTCATCTAACCTCACACCTAATAA	ATGGGTGATATTTTTTTGAAG	GTATYGTAGTAGGTTAYGGTTTAGGGTAGATTYGT	4°C ∞
FBXO40	FBXO40-F	FBXO40-R*	FBXO40-S	FBXO40	95°C 15 min
					94°C-66°C-72°C X 5 ∣30s
					94°C-63°C-72°C X 5 ∣30s
					94°C-60°C-72°C X 50 ∣30s
					72°C 10 min
	TTGAGTGTTGATAAATATAGGGGAAGGAA	CATCTACTTACACCTAAACCCTTAATT	AGGGGAAGGAAAAAG	TYGTAAGTTGAGTTTGGTTATATTGTTTTTTGTGATATYGTTTTATTTTTAATTTYGT	4°C ∞
MED12L	MED12L-F	MED12L-R*	MED12L-S2	MED12L	95°C 15 min
					94°C-59°C-72°C X 5 ∣30s
					94°C-57°C-72°C X5 ∣30s
					94°C-55°C-72°C X55 ∣30s
					72°C 10 min
	AGTTAGGTATTTGAAAGGAAAGTTTAT	ATCCAATCCTACTACTTTCTAACT	GGAAAGTTTATTTGGAAAGAT	TGGATYGTTTTAAATGTATATATTYGTATATTYGATTGAAGYG	4°C ∞
TSPAN17	TSPAN17-F	TSPAN17-R*	TSPAN17-S	TSPAN17	95°C 15 min
					94°C ∣30s
					62°C ∣30s X55
					72°C ∣30s
					72°C 10 min
	TGTGTTTTATTTTTTTTTTGGAATAGGT	ACCCAAACCTCATACTAATCC	AAAGTTGGAGTTTAATTTTAGTATT	TTTTATTTYGTYGTTTYGTTYGAT	4°C ∞
CMPK2	CMPK2-F	CMPK2-R*	CMPK2-S	CMPK2	95°C 15 min
					94°C ∣30s
					63.5°C ∣30s X55
					72°C ∣30s
					72°C 10 min
	GATGTTGGATTGTGGTAGGTAGA	AAACTTTTCCCTCTTCTTAAAATTACCCT	ATATTTTATTTTAGTGTAGGAAATT	TTAATTTGGTGGAAGAAAGGYGATTTYGAGGTGTTAATTTGGGYGA	4°C ∞
GRIK2	GRIK2-F	GRIK2-R*	GRIK2-S	GRIK2	95°C 15 min
					94°C-63.5°C-72°C X 5 ∣30s
					72°C 10 min
	TAGTAGTGTAGTAGAATAGGGTTTGGTAAA	AAAAAATCAATAAAAAACCCAACCTTAAT	GGTTTGGTAAAATTTTTGTTAG	TAAAGYGTTTTTTGTGTYGGGTTGTGGTTYGTTATYGATATTTYGTTYGT	4°C ∞
HCN1	HCN1-F	HCN1-R*	HCN1-S	HCN1	95°C 15 min
					94°C-68°C-72°C X 5 ∣30s
					94°C-66°C-72°C X 5 ∣30s
					94°C-64°C-72°C X 55 ∣30s
					72°C 10 min
	GAGGTTTTTGGGGTTTAGAAGAAGATTG	CCAAATACAAATTACCCTTCTTTTAGG	TTTTGGGGAGTGTGT	TTAGTTTGTTTTTTTTTATAGGYGTTGGTTATTTTYGTTGTGTTTATTTAGAAATAGYGGTYGG	4°C ∞
KLF16	KLF16-F	KLF16-R*	KLF16-S	KLF16	95°C 15 min
					94°C-63.5°C-72°C X 55 ∣30s
					72°C 10 min
	GGGGAGGGATGGGTTTGGA	CAACACAACTACATCCACCCCTAA	GGGAAGGTAGTATTGTTAT	TTYGGGATTGTAGTAAGAAATAGGTTTGGGGGTGTTTAGTYGG	4°C ∞
NR4A2	NR4A2-F	NR4A2-R*	NR4A2-S	NR4A2	95°C 15 min
					94°C-64°C-72°C X 5 ∣30s
					94°C-62°C-72°C X 5 ∣30s
					94°C-60°C-72°C X 5 ∣30s
					94°C-58°C-72°C X 55 ∣30s
					72°C 10 min
	GGGTTTAGGGGAAAGTGAAGT	ACTAACCCTAACCCCCAATATACCTTTAT	GTTAGGTAGGAAATATATTAAAG	YGAGYGYGGGTTAGGAGTTTAGGGAGYGYGG	4°C ∞