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. 2023 Mar 10;19(3):e1010843. doi: 10.1371/journal.ppat.1010843

Fig 5. Antiviral protection mediated by IFNε and other type-I IFNs is potently inhibited due to ZIKV inhibition of STAT1/2 signalling.

Fig 5

A & B) Ect1 cells were infected with ZIKV MOI of 10, 24 h post infection cells were stimulated with hIFNε (100 U/mL) for 30 min then fixed with acetone/methanol for detection of ZIKV E-antigen (red) and phosphorylated STAT1 or STAT2 proteins (green) by indirect immunofluorescence, DAPI (blue), infected cells (white arrows) and indicate uninfected bystanders (gold arrows). C & D) HeLa cells were infected at the indicated MOI of ZIKV 24h prior to stimulation with the either mIFNε (10 U/mL) or hIFNα-2A (500 U/mL) for 30 min, lysates were harvested for immunoblot of STAT1/2 protein and phosphorylated STAT1/2 proteins.