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. 2023 Mar 10;19(3):e1010843. doi: 10.1371/journal.ppat.1010843

Fig 6. Evasion of IFNε antiviral activity is mediated by ZIKV NS5 degradation of STAT2.

Fig 6

A & B) HeLa cells were transfected with pCDNA6.2-ZIKV-NS5-FLAG, NS2B/3-FLAG or empty vector control and 24 h later stimulated for 30min with the indicated IFN prior to assessing total and phosphorylated STAT1/2 by immunoblotting. C) ISRE promoter activity was assessed following 24 h IFNε stimulation in the presence or absence of ZIKV NS5A expression. D) HeLa cells were transfected with pCDNA-NS5-FLAG expression plasmid or an empty vector control (EV), 24h post transfection cells were stimulated with the indicated type-I IFN for 6 h prior to harvesting RNA for qRT-PCR analysis of ISG15 expression.