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. 2023 Mar 10;19(3):e1011214. doi: 10.1371/journal.ppat.1011214

Fig 2. Polymerase activity of polymerase gene segments carrying PB1 E177G, A652T and NP I119M and in vitro replication kinetics of viruses with these PB1 and NP substituations.

Fig 2

Activity of polymerase complexes carrying PB1 and NP substitutions in HEK-293T, A549, and SH-SY5Y cells (A). Polymerase activity is presented relative to that of H5N1-WT. Dotted lines indicate polymerase activity at H5N1-WT level and 2-fold increased activity. Mutant PB1 or NP genes that have significantly different polymerase or replicative (B) activity from that of H5N1-WT. Signifancies are indicated by asterisks, identified using a one-way (A) analysis of variance (ANOVA) with Bonferroni’s multiple-comparison tests or a two-way (B) analysis of variance (ANOVA) with Dunnett’s multiple-comparison tests. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001. Data are presented as means ±SEMs for panels A and ±SDs for panels B from at least three independent experiments.