Table 1. Mutations detected in the CNS of a ferret infected with virus of the H5N1 subtype.
Three substitutions were found in common between the cerebellum (CL) and cerebrospinal fluid (CSF). REF refers to the nucleotide reference; ALT to the nucleotide alternative variant; REF-AA amino acid reference; ALT-AA to substitution. Sequencing was done using MySeq Illumina platform.
Sample | Segment | Position | REF | ALT | REF-AA | ALT-AA | Frequency | Coverage |
---|---|---|---|---|---|---|---|---|
CL | PB1 | 554 | A | G | E | G | 0.994 | 5011 |
CL | PB1 | 1978 | G | A | A | T | 0.662 | 4683 |
CL | NP | 402 | T | G | I | M | 0.991 | 7980 |
CSF | PB1 | 554 | A | G | E | G | 0.993 | 7934 |
CSF | PB1 | 1978 | G | A | A | T | 0.534 | 7930 |
CSF | NP | 402 | T | G | I | M | 0.992 | 7986 |