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. 2023 Mar 2;12:e85464. doi: 10.7554/eLife.85464

Figure 5. Impaired B lymphopoiesis and myeloid skewing in Ogt cKO mice.

(A) Schematic view of B cell development in the BM and blockade by stromal OGT deficiency (red X). (B–C) Flow cytometric quantification of LSK (B) and CLP (C) among live BM cells (n=4–6). (D–I) Flow cytometric quantification of fraction A (D), fraction B (E), fraction C (F), fraction C’ (G), fraction D (H), and immature B (I) frequencies among live BM lymphocytes (n=6–7). (J–L) Flow cytometric quantification of B220+ B cell (J), CD4+ T cell (K), and CD8+ T cell (L) percentages in the blood (n=3–4). (M, N) CMP/CLP ratio (M) and GMP/EMP ratio (N) in the BM (n=6–7). (O, P) Complete blood counting showing numbers of RBC (O) and neutrophil (P) (n=7–9). Data are presented as mean ± SEM. *, p<0.05; **, p<0.01; and ***, p<0.001 by unpaired student’s t-test.

Figure 5—source data 1. Antibodies used for flow cytometry.

Figure 5.

Figure 5—figure supplement 1. Flow cytometry of BM B cells.

Figure 5—figure supplement 1.

(A) B cell lineage development and surface markers used for flow cytometry. (B) Gating strategy for B cell populations.