Figure 4. Enteroendocrine cell types that accelerate or slow gut transit.

Mice of genotypes indicated were injected with CNO (IP, 3 mg/kg) and gavaged orally with charcoal dye. Intestinal tissue was harvested, and the distance between the pyloric sphincter and the charcoal dye leading edge was measured. Representative images (left) and quantification (right) of gut transit. Scale bars: 1 cm, circles: individual mice, n: 5–14 mice, mean ± sem, *p<0.05, **p<0.01 by a Mann–Whitney test with Holm–Šídák correction. See Figure 4—figure supplement 1.

Figure 4—figure supplement 1. Supporting data for gut transit measurements.

(A) Mice indicated were injected with CNO (IP, 3 mg/kg), and gavaged orally with carmen red dye. Intestinal tissue was harvested and the distance between the pyloric sphincter and the dye leading edge measured. Representative images (left) and quantification (right) of gut transit, scale bar: 1 cm, circles: individual mice, n: 4–5 mice, mean ± sem, **p<0.01 by a Mann–Whitney test. (B) Native tdTomato fluorescence in fixed cryosections (20 μm) of tissues from mice indicated, scale bars: 100 μm. (C) Native reporter fluorescence was analyzed in wholemount tissue preparations indicated. Scale bars: 500 μm.