Figure 7.

Glutaraldehyde cross-linking of key korB deletion derivatives. His-tagged fragments of KorB polypeptide at a concentration of ∼0.3 mg/ml were incubated for 15 min with increasing concentrations of glutaraldehyde (0.001, 0.002, 0.005 and 0.01%) and then the samples were separated by SDS–PAGE on 20% Phast homogenous gels. Monomeric, dimeric and higher order complexes are indicated by m, d and h, respectively.