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Immunoprecipitation of native Rad51C from TK6 cells results in co-immunoprecipitation of the other four Rad51 paralogs, but not Rad51. A monoclonal Rad51C antibody was used for immunoprecipitation (right hand lanes), and normal mouse IgG was used as a negative control (left hand lanes). (A–F) Polyclonal antibodies specific for each Rad51 paralog and for Rad51 were used in western detection.
