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. 2022 Dec 23;72(4):520–531. doi: 10.2337/db22-0744

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© 2023 by the American Diabetes Association

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Regulation of LncCytB and nucleoids in mtDNA. A: Nucleoids in live cells were determined using SYBR Green for DNA and MTR for mitochondria. B: The number of nucleoids counted in five to eight images per group using ImageJ software. Measurements were made in duplicate/triplicate in three to four cell preparations, and the data are mean ± SD. HG/LnCyt, HG/LnCyt-si, HG/EV, and HG/SC indicate transfected cells with LncCytB-overexpressing plasmids, LncCytB-siRNA, empty vector, and SC RNA, respectively, in HG. *P < 0.05 vs. NG; #P < 0.05 vs. HG.