Figure 6.

Effect of diabetes on LncCytB expression and mtDNA damage and copy numbers. A: LncCytB transcripts were quantified in the retinal microvessels by strand-specific PCR using 18S as a housekeeping gene. B: mtDNA damage was assessed by extended length PCR using primers for long (10 kb) and short (116 bp) amplicons of mtDNA. C: mtDNA copy numbers were determined in the genomic DNA by quantifying the ratio of gene transcripts of mtDNA-encoded CYTB and nDNA-encoded 18S. Data are mean ± SD obtained from five to eight mice in each experimental group. *P < 0.05 vs. WT-N; #P < 0.05 vs. WT-D.