Figure 8.

Mitochondrial LncRNAs in the retinal microvessels from donors with documented diabetic retinopathy (DR) compared with age-matched donors without diabetes. A: Transcripts of LncCytB, LncND5, and LncND6 were quantified by RT-PCR using β-actin as a housekeeping gene. B: LncCytB transcripts were quantified by strand-specific PCR. C: FACS analysis was performed in the isolated mitochondria using SYBR Green-MTR dual staining. D: SYBR Green-MTR–positive cells in Q2 were plotted. Each measurement was made in duplicate with five to six donors per group. Data are mean ± SD, *P < 0.05 vs. age-matched donors without diabetes. Cont, unstained mitochondria.