Skip to main content
. 2002 Feb 15;30(4):975–982. doi: 10.1093/nar/30.4.975

Figure 4.

Figure 4

(A) The effect of added c-Fos or c-Jun on transactivation of the γD promoter by c-Maf. Explants were co-transfected with the –73/+10 γD–CAT construct and an expression construct for c-Maf (at 0.2 µg/transfection) and an expression construct for c-Fos or c-Jun using a decreasing DNA ratio to c-Maf DNA, as indicated. Results are expressed relative to the activity of the γD construct in cells without added Maf, which was set at 1. (B) The effect of dominant negative c-Fos or c-Jun on activity of the γD promoter. Explants were co-transfected with the –73/+10 γD–CAT construct and an expression construct for a dominant negative c-Fos (dn c-Fos) or c-Jun (dn c-Jun), as indicated. As a control, the same experiment was performed using a –73/+10 γD construct containing an AP1 site directly downstream of the MARE [γD(AP1); this mutant is described as γD4 in Klok et al. (12)]. Results are expressed relative to the activity of the γD construct in cells without added factors, which was set at 1.