Figure 7.

The effect of co-transfection of c-Maf on activity of the γD promoter in the presence or absence of growth factors. Lens explants were treated and transfected as described in the legend to Figure 6 except that the c-Maf expression construct was co-transfected at the amount indicated as the ratio to γD–CAT DNA (the amount of which was held constant) in the Figure. Results are expressed relative to the activity of the γD construct in cells with FGF-2 but without added c-Maf, which was set at 1. Note that the activity of the –73/+10 γD–CAT construct in the absence of FGF-2 is close to background and that the background was not substracted. Exact activation ratios cannot therefore be calculated from these data.