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. 2023 Mar 8;615(7953):652–659. doi: 10.1038/s41586-023-05791-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Estimation of nuclear genome size of V. faba Hedin/2 using flow cytometry. The histogram of relative nuclear DNA content was obtained after flow cytometric analysis of propidium-iodide stained nuclei of V. faba ‘Hedin/2’ and Secale cereale cv. Dankovske (2C = 16.19 pg), which served as the internal reference standard. The low level threshold was set to channel 20 on a 512-channel scale histogram of relative fluorescence intensity; all remaining fluorescence events were recorded. 2C nuclear DNA content of V. faba Hedin/2 was estimated at 26.36 pg (± 0.26 s.d.). b, k-mer based estimation of genome size using Hedin/2 raw HiFi sequencing data. c, k-mer spectrum plot produced by Merqury for the Hedin/2 genome assembly. K-mer multiplicity refers to the number of times a k-mer is found in sequencing reads. Colour corresponds to the number of times a k-mer is found in the assembly. d, k-mer spectrum plot produced by Merqury for the Tiffany genome assembly.