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. 2023 Feb 8;615(7953):728–733. doi: 10.1038/s41586-023-05779-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, The same consensus reconstruction map shown in Extended Data Fig. 2a. b, Focused classification with partial signal subtraction yielded 3D reconstruction maps with improved density quality of the bottom region and revealed two major conformational states as seen with 3D variability analysis (see Extended Data Fig. 2b). The mask used for focused classification is highlighted in yellow. Selected classes subjected to further processing are boxed in black. GSFSC plots are shown to the right of corresponding classes. See Methods for details.