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. 2023 Mar 15;615(7953):712–719. doi: 10.1038/s41586-023-05793-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — a, Representative of mitochondrial cristae structure in SBEM images of OXPHOS^HI LUAD (left panel) and OXPHOS^LO LUSC (right panel). The classification of type I, II and III cristae was indicated in the representative images. b–e, Representative of mitochondrial cristae structure of OXPHOS^HI LUAD (b,c) and OXPHOS^LO LUSC (d,e) cells imaged by confocal Airyscan and processed by WEKA segmentation (ImageJ). The classification of type I, II and III cristae was indicated in the representative images. Scale bar (b) = 3 μm. Scale bar (d) = 2 μm. f, Transmitted electron microscopy (TEM) image of OXPHOS^LO LUSC (RH2) cell. Zoomed-in images from red boxes illustrated type II cristae structure. Scale bar = 3 μm. g, Workflow of mitochondrial cristae segmentation and quantification in SBEM images by trainable WEKA segmentation (ImageJ). h, Mitochondrial cristae density (cristae number/mitochondrial area) in OXPHOS^HI LUAD and OXPHOS^LO LUSC cells imaged by SBEM (n = 20 mitochondria per tumor type). Data are mean ± s.e.m., unpaired two-tailed t-test. i, Mitochondrial cristae density (cristae number/mitochondrial area) in human OXPHOS^HI LUAD (H1975, A549) and OXPHOS^LO SCC (RH2, Tu686) cells. Data are mean ± s.e.m. (n = 2 biological replicates, >100 cells per cell line). j, Percentage of type II cristae distribution in human LUAD (H1975, A549) and SCC (RH2, Tu686) cells. Data are mean ± s.e.m. (n = 3 biological replicates, n > 2,000 mitochondria). Unpaired two-tailed t-test. k, Basal OCR of in human OXPHOS^HI LUAD (H1975, A549) and OXPHOS^LO SCC (RH2, Tu686) cells. Data are mean ± s.e.m. (n = 3 biological replicates). Unpaired two-tailed t-test.

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