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. 2023 Mar 16;29(3):632–645. doi: 10.1038/s41591-022-02178-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — a) Quantification of MM cells, CD4⁺ and CD8⁺ T lymphocytes, NK cells and T_reg cells in syngeneic MM5080 (n = 3-7) and control C57BL/6 (n = 3-6) mice is shown. b) Syngeneic transplants showed higher number of immunosuppressive PD-1⁺ Treg cells with respect to control C57BL/6 mice. c) Characterization of CD4⁺ T lymphocytes in syngeneic transplants (n = 4) vs. control C57BL/6 (n = 4) mice. d) Characterization of CD8⁺ T lymphocytes in syngeneic transplants (n = 4) vs. control C57BL/6 (n = 4) mice. e) Syngeneic transplants from the MM5080 cell line were refractory to therapies with moAbs that inhibit PD-1, PD-L1 and TIGIT. Therapy responses were determined by comparing median overall (mOS) in Kaplan-Meier survival curves. The number of mice included on each cohort is indicated. f) Simultaneous inhibition of PD-L1 and TIGIT moderately increased survival in a fraction of treated mice. Therapy responses were estimated by Kaplan-Meier survival curves. The number of mice included on each cohort is indicated. g) Depletion of T_reg cells with the anti-CD25 moAb combined with inhibition of PD-L1 efficacy decreased MM growth in the subcutaneous MM8273 syngeneic model. Boxes represent median, upper and lower quartiles and whiskers represent minimum to maximum range (a, b, c and d). P values obtained from two-tailed t tests (a, b, c and d), Mann-Whitney tests (a, b, c and d) and Kruskal-Wallis adjusted for multiple comparisons by Dunn’s test (g) are indicated. Log-rank (Mantel-Cox) test was used in e and f. *p < 0.05; **p < 0.01; ***p < 0.001; NS, not significant.