Extended Data Fig. 3. Influenza-induced hypothermia and sickness behaviors are attenuated by cell-specific Ptger3 knockout and aspirin.
a, Core body temperature in flox-Ptger3 and Gabra1-ires-Cre; flox-Ptger3 mice was measured daily after administration of influenza virus (red, blue) or saline (black) using a rectal probe (left) or radiotelemetry (right), mean ± sem, n: 6 (left) and 3 (right) mice per group, ***p < 0.0005 by one-way ANOVA Dunnett’s multiple comparison test (left); ***p < 0.0005 by two-tailed unpaired t-test for right as detailed in Fig. 1 for behavior/physiology analysis (right), comparisons between red and black curves (red stars) or between red and blue curves (blue stars). For data on the right, two of three control flox-Ptger3 mice died on day 5, so subsequent data is represented as a dashed line and statistical analysis was performed only on data from days 1-4. b, Mice were given daily injections of aspirin (IP, 20 mg/kg), red or vehicle alone (black) throughout the paradigm. After three days, mice were infected with influenza A virus and subsequently monitored as indicated, mean ± sem, n: 6 mice per group, ***p < 0.0005 by two-tailed unpaired t-test as detailed in Fig. 1 for behavior/physiology analysis, *p < 0.05 by a log-rank (Mantel-Cox) test for survival analysis. p values in a, left: red <0.0001, blue 0.0003, right: <0.0001; b, left to right: <0.0001, <0.0001, <0.0001, <0.0001, 0.0243.