Table 1.
Established tests on ready biodegradability
| Test | Method | Test principle | Remarks |
|---|---|---|---|
| DOC-die-away-test | OECD 301 A (1992), ISO 7827 (2010) | Static aerobic test system, measurement of DOC removal | Non-volatile water-soluble compounds |
| CO2 evolution test | OECD 301 B (1992), ISO 9439 (1999) | Static aerobic test system, measurement of CO2 production | Non-volatile water-soluble compounds |
| Continous CO2 evolution test | OECD 301 B (1992), ISO 9439 (1999) | Static aerobic test system, online measurement of CO2 production by conductivity measurement | Volatile/non-volatile water-soluble compounds, applied both as open and closed system (Strotmann et al. 2004) |
| Modified MITI (I) test | OECD 301 C (1992) | Static aerobic test, BOD determination, specific analysis possible | Non-volatile, water-soluble compounds; |
| Closed bottle test | OECD 301 D (1992), ISO 10707 (1994) | Static aerobic test, measurement of BOD | Volatile and toxic compounds at test concentrations of 2 mg L-1 |
| Modified OECD screening test | OECD 301 E (1992), ISO 7827 (2010) | Static, aerobic test, measurement of DOC removal | Non-volatile water-soluble compounds at Low inoculum concentration |
| Manometric respirometry test | OECD 301 F (1992), ISO 9408 (1999) | Static, aerobic test, measurement of BOD, and comparison to COD and ThOD of the test substance | Poorly water-soluble, non-volatile, and volatile compounds |
| CO2 headspace test | OECD 310 (2014), ISO 14593 (1999) | Static aerobic test, measurement of CO2 evolution | Volatile compounds, comparable to the CO2 evolution test |
| Biodegradability in seawater | OECD 306 H (1992), ISO 16221 (2001) | Static aerobic test system, measurement of DOC removal | Non-volatile water-soluble compounds, |