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. 2023 Mar 3;107(7-8):2073–2095. doi: 10.1007/s00253-023-12406-6

Table 2.

Inherent biodegradability tests

Test Method Test principle Remarks
Modified SCAS Test (Semi-continuous activated sludge) OECD 302 A (1981), ISO 9887 (1992) Semi-static, aerobic test system, fill- and draw method, measurement of DOC removal, test period up to 26 weeks Non-volatile, water-soluble compounds, pre-adaptation and specific analysis to determine primary biodegradation possible
Zahn-Wellens/EMPA Test OECD 302 B (1992), ISO 9888 (1999) Static, aerobic test system, high test compound, and inoculum concentration, measurement of DOC removal Non-volatile, water-soluble compounds
Modified MITI (II) Test OECD 302 C (1981) Static, aerobic test system, comparable to OECD 302 B (1992) but a specially prepared inoculum is required Non-volatile, water-soluble compounds
Inherent biodegradability in soil OECD 304 A (1981) Static, aerobic test, addition of 14C labeled test compound, determination of 14CO2 Closed system; volatile/non-volatile and soluble/non-soluble compounds