Table 3.

Simulation tests

Test	Method	Test principle	Remarks
Aerobic sewage treatment A: activated sludge units B: biofilms	OECD 303 A (2001), OECD 303 B (2001)	Static, aerobic test system, measurement of DOC or COD decrease	Non-volatile, water-soluble, or dispersible compounds
Aerobic and anaerobic transformation in soil	OECD 307 (2002)	Static aerobic/anaerobic test, use of 14C labeled compounds, measurement of 14CO2 formation	Volatile water-soluble and poorly water-soluble compounds
Aerobic and anaerobic transformation in aquatic sediment systems	OECD 308 (2002)	Static aerobic/anaerobic test, use of labeled/unlabeled compounds, analysis of original compound, and transformation products	Non-volatile and slightly volatile compounds
Aerobic mineralisation in surface water	OECD 309 (2004)	Static/semi-continuous aerobic test system, use of labeled (14C)/unlabeled compounds, determination of primary/ultimate biodegradation	Non-volatile/slightly volatile compounds. water-soluble/poorly water-soluble compounds
Simulation tests to assess the biodegradability of chemicals discharged in waste water	OECD 314 (2008) A: Biodegradation in a sewer system  OECD 314 (2008) B: Biodegradation in activated sludge test OECD 314 (2008) C: Biodegradation in anaerobic digester sludge test OECD 314 (2008) D: Biodegradation in treated effluent-surface water mixing zone test OECD 314 (2008) E: Biodegradation in untreated wastewater-surface water mixing zone test	Open/closed gas flow-through static systems, determination of primary/ultimate biodegradability, determination of transformation products, use of radiolabeled compounds recommended, but non labeled compounds permitted when an analytical procedure is given	All stages of wastewater treatment plant, volatile/non- volatile compounds, assessment of a mass balance