Table 4.

Other biodegradability tests and guidances

Test	Method	Test principle	Remarks
Anaerobic biodegradation test	OECD 311 (2006),  ISO 11734 (1995)	Static, anaerobic test system, measurement of biogas production (CH4/CO2), test duration up to 60 days, inoculum: anaerobic sludge	Compounds in concentrations of 20 - 100 mg L-1 organic carbon
Aerobic composting test	ISO 14855-1 (2012) (general method), ISO 14855-2 (2018) (gravimetric method for the determination of CO2 evolution)	Static aerobic test system, use of an adsorbing material (Vermiculite) possible, measure-ment of CO2 production or oxygen depletion, extended test duration, higher test temperature	Solid polymeric compounds
Biodegradation of polymers in aquatic environment	ISO 14851 (2019) (oxygen depletion) ISO 14852 (2021) (CO2 evolution)	Static aerobic test system, measurement of CO2 production or oxygen depletion, medium with a higher buffer capacity, extended test duration	Miscible and water soluble polymeric compounds
Low concentration tests in water	ISO 14592 (2002)	Guideline to perform biodegradation tests at very low concentrations
Guidance for poorly water-soluble compounds	ISO 10634 (2018)	Guideline to perform biodegradation tests with poorly water-soluble compounds
Guidance for selection of biodegradation tests	ISO 15462 (2006)	Tests in the aquatic environment