Table 4.
Other biodegradability tests and guidances
| Test | Method | Test principle | Remarks |
|---|---|---|---|
| Anaerobic biodegradation test | OECD 311 (2006), ISO 11734 (1995) | Static, anaerobic test system, measurement of biogas production (CH4/CO2), test duration up to 60 days, inoculum: anaerobic sludge | Compounds in concentrations of 20 - 100 mg L-1 organic carbon |
| Aerobic composting test | ISO 14855-1 (2012) (general method), ISO 14855-2 (2018) (gravimetric method for the determination of CO2 evolution) | Static aerobic test system, use of an adsorbing material (Vermiculite) possible, measure-ment of CO2 production or oxygen depletion, extended test duration, higher test temperature | Solid polymeric compounds |
| Biodegradation of polymers in aquatic environment | ISO 14851 (2019) (oxygen depletion) ISO 14852 (2021) (CO2 evolution) | Static aerobic test system, measurement of CO2 production or oxygen depletion, medium with a higher buffer capacity, extended test duration | Miscible and water soluble polymeric compounds |
| Low concentration tests in water | ISO 14592 (2002) | Guideline to perform biodegradation tests at very low concentrations | |
| Guidance for poorly water-soluble compounds | ISO 10634 (2018) | Guideline to perform biodegradation tests with poorly water-soluble compounds | |
| Guidance for selection of biodegradation tests | ISO 15462 (2006) | Tests in the aquatic environment |