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Identification of Syf1p, Syf2p and Syf3p as components of the Prp19p-associated complex. Syf1p, Syf2p and Syf3p were individually tagged with the HA epitope. Immunoprecipitation with the anti-HA antibody of extracts prepared from these strains (lanes 3, 4 and 6) revealed co-precipitation of the identified Prp19p-associated components.
