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. 2002 Feb 15;30(4):1029–1037. doi: 10.1093/nar/30.4.1029

Figure 3.

Figure 3

Figure 3

(A) Immunoprecipitation of the spliceosome formed in PRP19-HA (lanes 1–3), NTC90-HA (lanes 4–6), NTC77-HA (lanes 7–9) and NTC31-HA (lanes 10–12) extracts with the anti-HA antibody (lanes 3, 6, 9 and 12). (B) Immunoprecipitation of the spliceosome formed at various ATP concentrations in extracts prepared from a strain in which Prp19p was tagged with the c-Myc epitope and Prp4p tagged with the HA epitope. The splicing reaction was carried out at 0.5 mM (lanes 1–7), 0.1 mM (lanes 8–14) and 0.05 mM (lanes 15–21) ATP and the reaction mixtures (15 µl each) were subjected to immunoprecipitation with protein A–Sepharose alone (lanes 2, 9 and 16), anti-Myc (lanes 3, 10 and 17), anti-HA (lanes 4, 11 and 18), anti-Ntc90p (lanes 5, 12 and 19), anti-Ntc77p (lanes 6, 13 and 20) and anti-Ntc31p antibodies (lanes 7, 14 and 21). RXN, 2 µl of the reaction mixture; NS-Ab, non-specific antibody; PAS, protein A–Sepharose.