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. 2023 Mar 22;14:1573. doi: 10.1038/s41467-023-37217-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Quantitative RT-PCR analysis of STING mRNA expression in transfected B16-F10 cells with siRNA specific for DNMT3A (siDNMT3A) or DNMT3B (siDNMT3B) or nontarget siRNA (siControl) (n = 3). Data are shown as mean ± SD and are representative of two independent experiments. Statistical significance was determined using one-way ANOVA (a) (ns, not significant). Immunoblot analysis of STING, DNMT3A, and DNMT3B expression in indicated cells. β-Actin was used as a loading control (b). Levels of CXCL10 (c) and IFN-β (d) in cell culture supernatants measured using ELISA and reported as mean ± SD (n = 3 biological replicates). Data are representative of two independent experiments (c, d). Statistical significance was determined by one-way ANOVA. Immunoblot analysis of STING, DNMT3A and DNMT3B expression in B16-F10 (e) and A375 and SK-MEL-28 human melanoma cell lines (f) with or without 5AZADC treatment. Images in (b) and (e–f) are representative of three independent experiments. Correlative analysis of STING mRNA expression with DNMT3A and DNMT3B in metastatic melanoma samples (n = 21) from cBioPortal database using Pearson’s correlation coefficient. P-values shown are two-sided P-values derived from the Pearson correlation test (g).