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. 2023 Mar 22;13:4707. doi: 10.1038/s41598-023-31673-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Like SC-Ads, M-Ads rapidly die when cultured in 2D. (a) Representative phase-contrast images taken under light microscope of human SC-Ads and M-Ads cultured in 2D for the indicated times; Scale bar, 100 µm. (b) Representative images of BODIPY® 493/503 (neutral lipids, green) stained primary SC-Ads and M-Ads in 2D culture for the indicated times; Scale bar, 100 µm. (c,d) Number of SC-Ads (c) and M-Ads (d) cultured in 2D over time (n = 3). (e) Representative pictures of the culture wells of M-Ads grown in 2D at indicated times points. (f) Quantification of LDH release in medium during 2D culture at D2 and D7 for SC-Ads and M-Ads (n = 3). The results are expressed as the percentage of the value obtained when the whole cell population (same cell number than D0) is lysed. The histograms represent mean ± SEM, ns non-significant, *P < 0.05.