Table 1.
Statistics for twelve mcr-containing MAGs reconstructed from LCB metagenomes.
| MAG | Taxonomy | Size (Mbp) | Contigs | Genes | Cov. | GC (%) | Compl. (%) | Redund. (%) | Abund. (%) | tRNAs | rRNAs 16S,23S,5S | mcrA | Quality |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| LCB019-065 | Methanolinea | 1.78 | 27 | 1847 | 587.6 | 57.9 | 98.7 | 0 | 2.33 | 17 | 1,1,1 | 1 | Medium |
| LCB019-055 | Methanothermobacter | 1.55 | 217 | 1841 | 31.9 | 49.8 | 96.5 | 0.46 | 0.11 | 19 | 1,2,3 | 2 | High |
| LCB019-064 | Methanothrix | 1.66 | 72 | 1674 | 491.4 | 54.6 | 100 | 0 | 1.82 | 16 | 0,0,2 | 1 | Medium |
| LCB019-061 | Methanomassiliicoccales | 1.41 | 237 | 1501 | 24.3 | 44.3 | 87.61 | 3.23 | 0.08 | 13 | 1,0,1 | 1 | Medium |
| LCB019-004 | Ca. Methanomethylicia | 1.17 | 216 | 1433 | 33.8 | 53.7 | 91 | 4.52 | 0.09 | 11 | 0,0,0 | 3 | Medium |
| LCB019-019 | Ca. Methanosuratus | 1.09 | 182 | 1312 | 87.4 | 56.0 | 87.5 | 1.01 | 0.21 | 13 | 0,0,0 | 1 | Medium |
| LCB019-026 | Ca. Methanomethylovorales | 1.48 | 111 | 1638 | 200.3 | 48.8 | 99.1 | 1.25 | 0.66 | 17 | 0,2,1 | 1a | Medium |
| LCB024-024 | Ca. Methanomethylovorales | 1.41 | 221 | 1673 | 64.9 | 49.6 | 88.5 | 2.1 | 0.24 | 16 | 0,0,1 | 1a | Medium |
| LCB003-007 | Ca. Methanomethyloarchaeales | 1.03 | 67 | 1243 | 26.7 | 27.9 | 93.5 | 0 | 0.11 | 15 | 0,0,1 | 1 | Medium |
| LCB024-038 | Ca. Methanomethyloarchaeales | 1.17 | 53 | 1368 | 18.7 | 27.8 | 99.1 | 0.07 | 0.06 | 18 | 1,1,1 | 1 | High |
| LCB024-003 | Archaeoglobi | 1.16 | 179 | 1399 | 87.2 | 45.7 | 88.5 | 1.31 | 0.27 | 18 | 0,1,0 | 2 | Medium |
| LCB024-034 | Ca. Hadesarchaeia | 0.73 | 188 | 945 | 62.6 | 66 | 72.1 | 0.93 | 0.12 | 15 | 0,0,0 | 1 | Medium |
Taxonomy for MAGs was assigned using 18 marker proteins (SI Table 4). Average GC content, completeness, coverage, and redundancy were determined with CheckM. Total abundance was inferred based on the average coverage and size of each MAG relative to all MAGs in the metagenome.
aIndicates mcrA was split across two scaffolds. Quality was assessed according to ref. [81]. Cov., coverage; Compl., completeness; Redund., redundancy; Abund., total abundance.