Fig. 4. Kinetic studies of reaction of NAD⁺ with different concentrations of CORM-A1 (1 : 1, 2 mM or 200 μM; 7 : 1 1.4 mM; 11 : 1 2.2 mM). The 1 : 1 reaction (2 mM CORM-A1: 2 mM NAD⁺) was incubated at in PBS at 37 °C. At each time point, the reaction was either conducted at or diluted by 10-fold to 200 μM for UV measurements. Other reactions were conducted at 200 μM (NAD⁺) for UV-Vis detection. Reactions were monitored using UV-Vis at 340 nm.