Fig. 5. (A) Reaction of 2 mM NAD⁺ with CORM-A1 (1 : 1, 2 mM) incubated at 37 °C for 30 minutes. Reactions monitored and AUC determined using LC-MS/MS (right) and UV-Vis (left). (B) Reaction of 1 mM NAD⁺ with CORM-A1 (10 : 1, 10 mM) incubated at 37 °C for 15 minutes. Formation of NADH (μM) over 30 minutes by reaction of 200 μM NAD⁺ and CORM-A1 (11 : 1, 2.2 mM) monitored by LC-MS/MS (right) and UV-Vis (left), quantification done using AUC. Ratios are calculated using the average AUC from duplication experiments.