Fig. 7. Reactions of NAD⁺ with CO gas, CORM-A1 and other relevant boron species. (A) Reactions of 1 mM NAD⁺ with CO gas (2 mL), CORM-A1 (10 : 1, 10 mM), boric acid (10 : 1, 10 mM), or BH₃-THF (10 : 1, 10 mM) at 37 °C for 15 minutes. Reactions monitored and AUC determined using LC-MS/MS. Ratios are calculated using the average AUC from duplication experiments. (B) Reaction of 1 mM NAD⁺ with iCORM-A1 (10 : 1, 10 mM), boric acid (10 : 1, 10 mM) and NaBH₄ (10 : 1, 10 mM). Unreacted CORM-A1 (2 mM) provided as a control. Reactions were diluted to 200 μM after 15 minutes for UV-vis measurements.