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. 2023 Mar 9;14:1114596. doi: 10.3389/fimmu.2023.1114596

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Copyright © 2023 Zhang, Zhang, Liu, Wu, Wang, Li, Huang, Qiu and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of EE on hippocampus and corpus callosum in CCH mice. (A, C) Immunofluorescence staining of NeuN in hippocampus (n = 6), scale bar = 40 μm. (B, D–F) Western blot analysis of NeuN, BDNF, P-CREB, and CREB in hippocampus, (n = 3). (G, I) Immunofluorescence staining of MBP in corpus callosum, (n = 3), scale bar = 50 μm. (H, J, K) The result of electron microscopy, scale bar = 5 μm. (n = 3). (L, M) Western blot analysis of MBP in corpus callosum (n = 3). Values represent the mean ± s.d. ^aP < 0.05 vs. Sham; ^bP < 0.05 vs. CCH group.